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. 2024 Jan 29;15:869. doi: 10.1038/s41467-024-45238-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — TUNEL assays on 5-day old Ae. aegypti dissected reproductive tissues revealed DNA breaks occurring in β2t-cifA-cifB testes which was inhibited by additional expression of cifA (β2t-cifA;β2t-cifA-cifB) whilst lowering the overall dosage of the Cifs (topi-cifA-cifB) also resulted in a lack of DNA breaks. Although wAlbB is predicted to produce two sets of nuclease CifB peptides no DNA damage was not observed in wAlbB-carrying testes. Scale bar, 50 µm.