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GC-FID separation of fatty acid methyl esters of P. putida NCTC 10936 in a nonpolar column (HP Ultra2). Cells were grown on 0.2% succinate for 8 h. (A) The sample was analyzed immediately; (B) the sample was resuspended in imidazole buffer at 30°C for 3 h before analysis. Peaks representing 16:1 and 16:0 are enlarged.
