FIG. 4.

Fatty acid compositions (wt/wt) of P. putida strains (P8 [A], NCTC 10936 [B], and KT 2440 [C]) grown on 0.2% succinate at 30°C. Groups of straight-chain saturated, cyclopropane, cis, and trans fatty acids are shown. Samples were harvested after 8 h (exponential growth phase) or 48 h (stationary growth phase), washed rapidly, and then frozen (−20°C). After thawing (30°C, 15 min), the samples were analyzed immediately (control, 0 h) or after an incubation time of 1 or 5 h in a nutrient-free buffer at 30°C. Representative results are shown. At least two independent experiments were performed (SEM, <5%).