FIG. 5.

Development of straight-chain saturated, cyclopropane, cis, and trans fatty acids of P. putida KT 2440 grown for 4 h on 0.2% succinate at 30°C. Samples were harvested, washed rapidly, and analyzed immediately after the following alternate treatments: 1, control sample without further treatment; 2, one freeze (−20°C)-thaw (30°C, 1 min) cycle; 3, two freeze-thaw cycles without a longer dwell time; 4 to 7, a freeze (−20°C)-thaw (10°C) cycle followed by a 2-h incubation at 10, 20, 30, and 40°C, respectively. At least two independent experiments were performed (SEM, <5%).