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. 2024 Jan 30;14:2441. doi: 10.1038/s41598-024-52337-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spatial reaction of glial cells after CHI and reduction of lesion size over time. (a) IHC shows the progressive accumulation of Iba1⁺ cells around and within the trauma site (the borders of the trauma site are highlighted by the pink dotted line in the left panel), while NG2-glia and GFAP⁺ astrocytes surround the lesion. Yellow dotted squares show the position of the higher-magnification images (right panels), used to highlight the colocalization of the different markers. (b) No significant reduction in the primary trauma dimension per slice is observed over time (one-way ANOVA with Tukey's post hoc test). Data are presented as the mean ± SEM.