TABLE 2.
Comparison of the real-time PCR results for DNA extracted from biofilms by four methods
| DNA extraction method |
CT ± SDa
|
P valueb | |
|---|---|---|---|
| Adherent cellsc | Nonadherent cells | ||
| Phenol-chloroform (3) | 19.27(A) ± 0.91 | 25.47 ± 1.70 | 0.001 |
| DNeasy tissue kit (Qiagen) | 20.94(AB) ± 0.81 | 23.53 ± 1.29 | 0.014 |
| Boiling (4) | 21.71(AB) ± 1.46 | 28.75 ± 2.87 | 0.004 |
| Potassium acetate (23) | 22.70(B) ± 2.58 | 31.28 ± 1.77 | 0.001 |
a
Values are means of four determinations.
b
Results of a Student t test for comparison of the means between DNA extraction methods for adherent and nonadherent biofilms.
c
Means with no common letters are statistically different at the 0.05 significance level according to Fisher's least significant difference procedure.