Skip to main content
. 2023 Jun 23;11(3):100988. doi: 10.1016/j.gendis.2023.05.005

Figure 5.

Fig. 5

Knockdown of LETM1 improved the function of mitochondria, inhibited the PPP, and promoted the OXPHOS in MG63 and 143B cells. Cells were infected with adenovirus for 72 h, and then (A) the α-KGDH activity assay kit was used to detect and compare the α-KGDH activity in the ADCON and sh-LETM1 groups. (B) The ICDHm activity assay was used to detect the ICDHm activity, which was compared between the ADCON and sh-LETM1 groups. (C) The total ATP content in the ADCON and sh-LETM1 groups was determined using an ATP assay kit. (D) The glucose uptake was determined by subtracting the amount of glucose present from the amount originally contained in the culture medium, divided by the protein level. (E) Detection of the intracellular glucose content was determined after cell lysis. (F) The lactic acid levels were measured and calculated using a lactic acid assay kit. (G, H, J) The mRNA expression levels of IDH1, IDH2, HK1, PFKM, PKM2, and LDHA were analyzed by real-time PCR. (I, K) The expression levels of the G6PD and PKM2 proteins were detected by Western blotting. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.