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. 2005 Apr;71(4):2140–2144. doi: 10.1128/AEM.71.4.2140-2144.2005

TABLE 3.

Comparison of the β-galactosidase activities of four randomly picked clones from the library and corresponding sequences of the lacZ upstream regions

Transformant Artificial promoter Start codon RBS sequence β-Galactosidase activity (U/mg)
1 1.6 GI TTG TCACAGGAGA 0.28 ± 0.1
2 1.6 GI ATG AAGGAGGAAA 5.7 ± 1.2
3 1.2 GI ATG ACACAGGAAA 0.7 ± 0.3
4 1.6 GI TTG ACACAGAAGA 0.03 ± 0.01