TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype and/or genotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH10B | Cloning strain | Gibco BRL |
| E. coli GEc137(pGEc47ΔB) | DH-1 thi fadR | 29 |
| P. putida GPo12(pGEc47ΔB) | P. putida GPo1 cured of the OCT plasmid | 29 |
| Plasmids | ||
| pGEc47ΔB | Contains all genes necessary for growth on n-alkanes except an alkane hydroxylase gene | 29 |
| pCom8 | Broad-host-range expression vector with PalkB, GmroriT alkS | 31 |
| pCom8-PFR1500 | P450 operon in pCom8 | This study |
| pCR2.1-TOPO | PCR cloning vector | Invitrogen |
| TOPO-HXN-1500 | pCR2.1-TOPO with HXN-1500 PCR fragment | This study |
| pKKPalk | E. coli expression vector with PalkB, Apr | 31 |
| pZErO2.1 | Cloning vector, Kmr | Invitrogen |
| pZErO2.1-Apa6.1 | pZErO2.1 with 6.1-kb ApaI fragment | This study |