TABLE 2.
Primers to investigate the IS insertion site on pGEN500
| Primer | Sequence (5′ to 3′) | Primer-annealing regiona |
|---|---|---|
| sacB-F1 | GATTTTTTAGTTCTTTAGGCC | −311 to −291 |
| sacB-R5 | GTAAAGGTTAATACTGTTGC | 47 to 28 |
| sacB-F2 | AACATCAAAAAGTTTGCAAAAC | 4 to 25 |
| sacB-R4 | TCATTTGCATCGAATTTGTC | 458 to 439 |
| sacB-F3 | GCCGCGTCTTTAAAGACAGC | 419 to 438 |
| sacB-R3 | CGTTTAGCTCAATCATACCG | 939 to 958 |
| sacB-F4 | TTAGCAAACGGCGCTCTCGG | 922 to 941 |
| sacB-R2 | TTATTTGTTAACTGTTAATTGTCC | 1422 to 1399 |
a
The numbers indicate the primer-annealing region on pGEN500. The first A residue of the sacB start codon was defined as position +1.