TABLE 3.
IS transposition frequency under several growth conditions
| Condition and experiment no. | TET-resistant cells/ml of culture (A) | Sucrose-resistant clones/ml of culture (B) | Ratio of IS-mediated-sucrose-resistant clones among 32 sucrose-resistant clones (C) | IS transposition frequencya (B × C/A) | Average | Standard deviation |
|---|---|---|---|---|---|---|
| Normal | ||||||
| 1 | 2.4 × 109 | 2.9 × 104 | 9/32 | 3.4 × 10−6 | 2.1 × 10−6 | 1.3 × 10−6 |
| 2 | 3.4 × 109 | 2.5 × 104 | 9/32 | 2.1 × 10−6 | ||
| 3 | 3.7 × 109 | 2.8 × 104 | 3/32 | 7.0 × 10−7 | ||
| Oxidative | ||||||
| 1 | 2.8 × 109 | 2.1 × 104 | 6/32 | 1.5 × 10−6 | 2.3 × 10−6 | 1.3 × 10−6 |
| 2 | 3.6 × 109 | 2.7 × 104 | 7/32 | 1.6 × 10−6 | ||
| 3 | 2.7 × 109 | 2.5 × 104 | 13/32 | 3.8 × 10−6 | ||
| High temperature | ||||||
| 1 | 3.8 × 109 | 1.1 × 105 | 9/32 | 8.6 × 10−6 | 1.6 × 10−5 | 7.2 × 10−6 |
| 2 | 3.8 × 109 | 1.9 × 105 | 10/32 | 1.5 × 10−5 | ||
| 3 | 2.1 × 109 | 1.3 × 104 | 12/32 | 2.3 × 10−5 | ||
| Starvation | ||||||
| 1 | 2.0 × 109 | 5.2 × 103 | 10/32 | 8.1 × 10−7 | 1.6 × 10−6 | 1.9 × 10−6 |
| 2 | 1.8 × 109 | 4.4 × 103 | 5/32 | 3.8 × 10−6 | ||
| 3 | 2.0 × 109 | 5.5 × 104 | 4/32 | 3.1 × 10−7 |
a
The number of sucrose- and TET-resistant clones per milliliter of culture (B) was multiplied by the ratio of IS-mediated-sucrose-resistant clones among 32 sucrose-resistant clones (C) and then divided by the number of TET-resistant cells per milliliter of culture (A). For each condition, three independent experiments were performed (experiments 1 to 3), and the average and standard deviation were calculated.