FIG. 3.

Alignment of the UMB primers with the corresponding regions of nifH from the γ-proteobacterium V. natriegens (top). The nucleotide positions where the V. natriegens sequence differs are indicated by the corresponding bases below the UMB sequences. The analytical agarose gel shows the PCR products obtained from either V. natriegens or the UMB clone 01/11/2O with either the universal nested primer set (U) or UMB primers (C) (bottom). The same template concentrations were used for each reaction.