TABLE 2.

Decolorization of five dyes after different treatment times with laccases from P. cinnabarinus and T. villosa using different acetosyringone/dye molar ratios^a

Dye and treatment time	% Decolorization at indicated acetosyringone/dye ratio
	P. cinnabarinus laccase				T. villosa laccase
	0	1	2	4	0	1	2	4
Azure B
5 min	0	14	23	29	0	17	24	34
30 min	0	18	28	39	0	20	30	41
2 h	0	16	26	33	0	17	26	36
Reactive Black 5
5 min	0	84	85	84	0	84	85	84
30 min	0	86	86	85	0	85	85	84
2 h	0	87	86	85	0	87	87	84
Acid Blue 74
5 min	2	100	100	100	3	100	100	100
30 min	7	100	100	100	8	100	100	100
2 h	25	100	100	100	24	100	100	100
Reactive Blue 19
5 min	17	67	64	57	21	64	61	57
30 min	44	68	64	50	53	66	60	50
2 h	78	69	63	47	83	67	59	47
Aniline Blue
5 min	2	43	66	77	3	40	63	78
30 min	3	47	68	81	5	43	66	81
2 h	10	53	72	82	13	49	69	82

^aDecolorization was monitored at the absorption maxima of Azure B (647 nm), Reactive Black 5 (598 nm), Reactive Blue 19 (592 nm), and Aniline Blue (592 nm).