TABLE 2.
Dye and treatment time | % Decolorization at indicated acetosyringone/dye ratio
|
|||||||
---|---|---|---|---|---|---|---|---|
P. cinnabarinus laccase
|
T. villosa laccase
|
|||||||
0 | 1 | 2 | 4 | 0 | 1 | 2 | 4 | |
Azure B | ||||||||
5 min | 0 | 14 | 23 | 29 | 0 | 17 | 24 | 34 |
30 min | 0 | 18 | 28 | 39 | 0 | 20 | 30 | 41 |
2 h | 0 | 16 | 26 | 33 | 0 | 17 | 26 | 36 |
Reactive Black 5 | ||||||||
5 min | 0 | 84 | 85 | 84 | 0 | 84 | 85 | 84 |
30 min | 0 | 86 | 86 | 85 | 0 | 85 | 85 | 84 |
2 h | 0 | 87 | 86 | 85 | 0 | 87 | 87 | 84 |
Acid Blue 74 | ||||||||
5 min | 2 | 100 | 100 | 100 | 3 | 100 | 100 | 100 |
30 min | 7 | 100 | 100 | 100 | 8 | 100 | 100 | 100 |
2 h | 25 | 100 | 100 | 100 | 24 | 100 | 100 | 100 |
Reactive Blue 19 | ||||||||
5 min | 17 | 67 | 64 | 57 | 21 | 64 | 61 | 57 |
30 min | 44 | 68 | 64 | 50 | 53 | 66 | 60 | 50 |
2 h | 78 | 69 | 63 | 47 | 83 | 67 | 59 | 47 |
Aniline Blue | ||||||||
5 min | 2 | 43 | 66 | 77 | 3 | 40 | 63 | 78 |
30 min | 3 | 47 | 68 | 81 | 5 | 43 | 66 | 81 |
2 h | 10 | 53 | 72 | 82 | 13 | 49 | 69 | 82 |
Decolorization was monitored at the absorption maxima of Azure B (647 nm), Reactive Black 5 (598 nm), Reactive Blue 19 (592 nm), and Aniline Blue (592 nm).