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. 2024 Jan 3;10(2):e24010. doi: 10.1016/j.heliyon.2024.e24010

Fig. 4.

Fig. 4

TP suppresses pyroptosis via activating Nrf2/HO-1 pathway (A–C) Western blot analysis of Nrf2 and HO-1 protein expression in mouse heart tissue (n = 6). (D–F) Western blot analysis of Nrf2 and HO-1 in HL-1 cardiomyocytes (n = 6). (G–H) Western blot analysis of Nrf2 expression in the presence or absence of Nrf2 siRNA in HL-1 cardiomyocytes (n = 6). (I–J) HO-1 expression elicited by AngⅡ in the presence or absence of Nrf2 siRNA in HL-1 cardiomyocytes (n = 6). (K–L) The concentrations of IL-1β and IL-18 in the presence or absence of Nrf2 siRNA in HL-1 cardiomyocytes (n = 3). (M–P) Western blot analysis of NLRP3, cleaved Caspase-1 and GSDMD expression in the presence or absence of Nrf2 siRNA in HL-1 cardiomyocytes (n = 6). (Q) Representative immunofluorescent staining of the cardiomyocytes (HL-1, α-actinin, red) and nuclei (DAPI, blue) elicited by AngⅡ in the presence or absence of Nrf2 siRNA in HL-1 cardiomyocytes. Scale bar: 10 μm (n = 6). (R) Relative surface area of HL-1 cardiomyocytes (n = 6). (S–U) Western blot analysis of β-MHC and BNP expression in the presence or absence of Nrf2 siRNA in HL-1 cardiomyocytes (n = 6). All the values are presented as mean ± SD. #P < 0.05, ##P < 0.01 compared with Ctl group; *P < 0.05, **P < 0.01 compared with siRNA-NC group or AngⅡ group; &P < 0.05, &&P < 0.01 compared with AngⅡ+TP + scRNA group. The original blots were provided in supplementary data as Supplementary Fig. S3.