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. 2024 Jan 3;10(2):e24010. doi: 10.1016/j.heliyon.2024.e24010

Fig. 7.

Fig. 7

Triptolide activates Nrf2 signaling by decreasing ubiquitin-proteasome degradation of Nrf2. (A) 3-dimensional chemical structures of Triptolide. (B–D) Western blot analysis of USP14 and Keap1 in mouse heart tissue (n = 6). (E–G) Western blot analysis of USP14 and Keap1 in mouse cardiomyocytes (HL-1) (n = 6). (H–J) Western blot analysis of USP14 and Keap1 in EN (HCAEC) (n = 6). (K–L) Western blot analysis of USP14 expression in the presence or absence of USP14 siRNA in mouse cardiomyocytes (HL-1) (n = 6). (M–N) Western blot analysis of USP14 expression in the presence or absence of USP14 siRNA in EN (HCAEC) (n = 6). (O–Q) Keap1 and Nrf2 expression elicited by AngⅡ in the presence or absence of USP14 siRNA in mouse cardiomyocytes (HL-1) (n = 3). (R–T) Keap1 and Nrf2 expression elicited by AngⅡ in the presence or absence of USP14 siRNA in EN (HCAEC) (n = 3). (U and V) The mouse cardiomyocytes (HL-1) and EN (HCAEC) was taken for Co-IP. Western blotting showed that Keap1 interacts with USP14. The original blots were provided in supplementary data as Supplementary Fig. S6.