Presence of binary ICPs in B. thuringiensis isolates identified by cry35 nucleic acid hybridization. Lanes: 1, molecular mass markers; 2, PS149B1; 3, PS223L2; 4, KB10H-5; 5, KB59A54-4; 6, KB59A54-5; 7, KB59A58-4; 8, KB65A14-1; 9, PS201V2; 10, PS207C3; 11, KR1369; 12, PS137A; 13, PS147U2; 14, KB65A15-2; 15, KB65A15-3; and 16, KB65A15-7. The 50% lethal concentration (LC50) (in micrograms per squared centimeters) or N (indicating a dose response could not be calculated) is indicated below each lane. A (−) indicates the absence of a 14-kDa protein.