FIG. 8.

Sequence homologies to Cry34. (A) Non-Bacillus sequences in GenBank showing homology to Cry34Ab1 in PSI-BLAST searches. Source organisms and protein accession numbers are indicated. The protein sequence is indicated by a horizontal line, with blackened ovals indicating the position of Cry34 similarity; significant gaps are indicated by segmentation, and white ovals indicating a phosphatase conserved domain (CDD entries COG5555, KOG1378, and KOG2679) for NP_929487 and a endonuclease/exonuclease/phosphatase domain (CDD entry pfam03372) for AAO52140. Truncated ovals indicate incomplete alignment of the conserved domain to the respective sequence. (B) Multiple-sequence alignment of unique Cry34 sequences and homologues. Black highlighting indicates conserved identity or similarity across all sequences, and dark and light gray shading indicates lesser degrees of conservation among the sequences. Consensus similarity groups are the following: 3-S,T and 6-L,I,V,M. The two different alignments with the D. discoidium sequence (accession no. AAO52140) are indicated by the start positions chosen for this alignment based on the PSI-BLAST alignment.