TABLE 2.
Microbial dynamics, as determined by plating, of the fermentations monitored in this study
| Microbiological analysis parameter | Result (log10 CFU/g) for fermentation at day givena | |||||||
|---|---|---|---|---|---|---|---|---|
| Fermentation C | 0 | 3 | 10 | 20 | 30 | 60 | 90 | 120 |
| Total aerobic count | 4.46 (0.27) | 4.49 (0.04) | 7.03 (0.88) | 6.48 (0.41) | 6.97 (0.56) | 7.24 (0.26) | 7.11 (0.03) | 7.02 (0.02) |
| CNC | 3.23 (0.10) | 4.70 (0.63) | 5.37 (0.80) | 5.72 (0.16) | 5.09 (0.12) | 3.58 (0.16) | 4.30 (0.12) | 5.20 (0.21) |
| LAB | 3.17 (0.93) | 3.74 (0.07) | 6.37 (0.69) | 7.83 (0.02) | 7.58 (0.12) | 7.28 (0.50) | 7.87 (0.51) | 8.78 (0.21) |
| Yeasts | 2.00 (0.00) | 3.12 (0.46) | 5.36 (0.02) | 5.90 (0.14) | 4.98 (0.25) | 2.50 (0.08) | 3.12 (0.09) | 6.73 (0.05) |
| Molds | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) |
| Fecal enterococci | 2.15 (0.27) | 2.40 (0.17) | 5.33 (0.33) | 7.18 (0.11) | 6.76 (0.07) | 6.81 (0.03) | 3.65 (0.15) | 4.12 (0.20) |
| Total enterobacteria | 1.73 (0.16) | 2.16 (0.43) | 3.20 (0.42) | 2.98 (0.58) | 1.97 (0.09) | 1.98 (0.02) | <10 (NA) | <10 (NA) |
| E. coli | 1.25 (0.24) | 1.43 (0.22) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) |
| S. aureus | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) |
| L. monocytogenes | A | A | A | A | NP | NP | NP | NP |
| Salmonella spp. | A | A | A | A | NP | NP | NP | NP |
| Fermentation L | 0 | 3 | 10 | 20 | 30 | 45 | ||
| Total aerobic count | 4.74 (0.44) | 3.59 (0.36) | 8.03 (0.56) | 7.68 (0.14) | 8.55 (0.30) | 4.19 (0.23) | ||
| CNC | 3.11 (0.18) | 3.65 (0.15) | 6.83 (0.07) | 6.12 (0.71) | 5.86 (0.06) | 4.63 (0.20) | ||
| LAB | 5.29 (0.18) | 5.97 (0.27) | 7.81 (0.21) | 7.94 (0.12) | 7.93 (0.16) | 8.34 (0.14) | ||
| Yeasts | 3.55 (0.80) | 2.93 (0.05) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | ||
| Molds | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | ||
| Fecal enterococci | 3.18 (0.12) | 4.47 (0.77) | 5.95 (0.15) | 4.95 (0.96) | 4.00 (0.00) | 5.47 (0.47) | ||
| Total enterobacteria | 1.94 (0.15) | 3.59 (0.36) | 4.29 (0.21) | 3.80 (0.66) | 4.28 (0.21) | 2.56 (0.09) | ||
| E. coli | 1.72 (0.63) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | ||
| S. aureus | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | ||
| L. monocytogenes | A | A | A | A | NP | NP | ||
| Salmonella spp. | A | A | A | A | NP | NP | ||
| Fermentation U | 0 | 3 | 5 | 7 | 14 | 28 | ||
| Total aerobic count | 5.09 (0.20) | 5.41 (0.22) | 5.88 (0.19) | 5.33 (0.34) | 6.90 (0.56) | 9.11 (0.47) | ||
| CNC | 3.66 (0.10) | 3.80 (0.63) | 5.67 (0.57) | 4.62 (0.69) | 5.54 (0.06) | 5.01 (0.12) | ||
| LAB | 4.20 (0.17) | 8.24 (0.01) | 7.81 (0.10) | 8.21 (0.03) | 8.28 (0.16) | 8.45 (0.06) | ||
| Yeasts | 3.33 (0.06) | <100 (NA) | <100 (NA) | <100 (NA) | 3.53 (0.68) | 2.32 (0.28) | ||
| Molds | 2.59 (0.26) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | ||
| Fecal enterococci | 3.73 (0.38) | 5.60 (0.00) | 5.83 (0.22) | 6.02 (0.16) | 6.08 (0.17) | 6.28 (0.32) | ||
| Total enterobacteria | 2.38 (0.06) | 4.94 (0.21) | 4.88 (0.38) | 2.18 (0.15) | 4.70 (0.41) | 4.66 (0.50) | ||
| E. coli | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | <10 (NA) | ||
| S. aureus | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | <100 (NA) | ||
| L. monocytogenes | A | A | A | A | A | NP | ||
| Salmonella spp. | A | A | A | A | A | NP | ||
a
Results are shown as the mean with standard deviation in parentheses. NA, not applicable; NP, not performed; A, absent in 25 g of product.