TABLE 1.
Bacterial strains and plasmidsa
| Strain or plasmid | Relevant characteristics and description | Reference or source |
|---|---|---|
| Strains | ||
| C. acetobutylicum | ||
| ATCC 824 | Wild type | American Type Culture Collection, Manassas, Va. |
| E. coli | ||
| DH5α | mcrA, ΔmcrBC, recA1 | NEB, Beverly, Mass. |
| DH10β | mcrA, ΔmcrBC, recA1, Strr | NEB |
| TOP10 | mcrA, ΔmcrBC, recA1, Strr | Invitrogen |
| Plasmids | ||
| pDHKM | RSF1030ori, Kmr, Φ3tI | 47 |
| pTrcHisTOPO-TA | Apr, pBR322ori | Invitrogen |
| pTrc310pro | Apr, pBR322ori, abrB310 promoter | This study |
| pTrc1941pro | Apr, pBR322ori, abrB1941 promoter | This study |
| pTrc3647pro | Apr, pBR322ori, abrB3647 promoter | This study |
| pCATP | MLSr, repL, ColE1ori, catP | 38 |
| pCAT310 | MLSr, repL, ColE1ori, catP, abrB310 promoter | This study |
| pCAT1941 | MLSr, repL, ColE1ori, catP, abrB1941 promoter | This study |
| pCAT3647 | MLSr, repL, ColE1ori, catP, abrB3647 promoter | This study |
| pIMP1 | Apr, MLSr, ColE1ori, repL | 26 |
| pM310 | Apr, MLSr, ColE1ori, repL, abrB310 | This study |
| pMsinR | Apr, MLSr, ColElori, repL, sinR | This study |
| pSOS94 | ptb promoter, ctfA-ctfB, adc Apr, MLSr, ColE1ori, repL | 45 |
| pASsos | ptb promoter, Apr, MLSr, ColElori, repL | This study |
| pASsin | ptb promoter, sinRAS, Apr, MLSr, ColElori, repL | This study |
| pAS310 | ptb promoter, abrB310AS, Apr, MLSr, ColElori, repL | This study |
a
MLSr, macrolide-lincosamide streptogramin B resistant; mcrA, ΔmcrBC, methylcytosine-specific restriction system abolished; recA1, homologous recombination abolished; Strr, streptomycin resistant; RSF1030ori, gram-negative origin of replication; Kmr, kanamycin resistant; Φ3tI, Φ3t methylase; pBR322ori, gram-negative origin of replication; Apr, ampicillin resistant; repL, gram-positive origin of replication; ctfA-ctfB, coenzyme A transferase subunits A and B; adc, acetoacetate decarboxylase; sinRAS, sinR antisense construct; abrB310AS, sinR antisense construct; catP, promoterless CAT open reading frame.