Figure 6.

PP2A activity and expression of Toll-1 and Toll-7 receptors are required for autophagy and DA neuron survival in Drosophila brain

(A‒C) Detection of P62 protein and DA neurons in Drosophila adult brain.

(D) Schematic diagram of the PAM region in Drosophila adult brain. Brains from the control flies (w¹¹¹⁸), Elav-Gal4 driven Toll-1 and Toll-7 RNAi flies, and Wdb^DN flies which constitutively express an inactive PP2A subunit were dissected and fixed, P62 protein in the brain was detected with anti-Ref(2)P antibody (A, a1-a4) and DA neurons were labeled with anti-TH antibody (A, b1-b4), then confocal images were taken. Scale bar: 50 μm in a1 to a4 and b1 to b4, 20 μm in the amplified sections. P62 fluorescence intensity was quantified (B) and DA neurons were counted (C) in the PAM region (D) of at least 20 brains for each fly line by ImageJ. Data were represented as means ± SEM. Significant difference was determined by one way ANOVA (see Figure 1 legend).