FIG. 2.
IL-10 production induced by B. burgdorferi in PBMC from uninfected rhesus monkeys. PBMC (3 × 106/ml) from uninfected rhesus monkeys were incubated with supplemented medium (RPMI), heat-killed spirochetes of the JD1 strain (106 spirochetes/ml), or ConA (8 μg/ml) for 48 h. IL-10 in the supernatant was determined by ELISA. Values are means of duplicate determinations. Each point represents the concentration of IL-10 in the culture supernatant of PBMC from an individual monkey, and the horizontal lines indicate arithmetic means. The lower limit of detection of the ELISA was 15 pg/ml.