FIG. 5.
IL-10 mRNA expression induced by B. burgdorferi M297 in PBMC from uninfected rhesus monkeys. PBMC (3 × 106/ml) from uninfected rhesus monkeys were stimulated with heat-killed spirochetes of the mutant strain M297 (106 spirochetes/ml) or ConA (8 μg/ml) for 24 h. The induced mRNA levels of IL-10 were determined by RT-PCR. Responses are shown as fold increases over unstimulated PBMC for each monkey. Each point represents the response of PBMC from an individual monkey, and the horizontal lines indicate geometric means. All values were normalized with respect to GAPDH mRNA levels.