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. 2023 Dec 22;6(1):e220127. doi: 10.1148/rycan.220127

Figure 3:

Whole-brain intracellular pH (pHi) images in participants with high- and low-grade glioma obtained using phosphorous 31 (31P) 7.0-T spectroscopic MRI. (A, B) Clinical (3.0 T) contrast-enhanced T1-weighted magnetization-prepared rapid-acquisition gradient-echo images with overlays of the volumetric three-dimensional (3D) color-coded pHi map in all three planes in a 75-year-old man (A, study participant 8 in Table 1) and a 68-year-old man (B, study participant 3 in Table 1), both with histopathologically proven glioblastoma (World Health Organization [WHO] grade IV). Axial (A1, B1), sagittal (A2, B2), and coronal (A3, B3) planes show an alkaline shift of pHi in the whole-tumor volume (A, pHi = 7.106 ± 0.045; B, pHi = 7.080 ± 0.059) compared with normal-appearing white matter (A, pHi = 6.996 ± 0.002; B, pHi = 6.993 ± 0.004). (C) Clinical (3.0 T) fluid-attenuated inversion recovery images with overlays of the volumetric 3D color-coded pHi maps in a 34-year-old man (study participant 10 in Table 1) with histopathologically proven astrocytoma (WHO grade II) in axial (C1), sagittal (C2), and coronal (C3) planes. No pronounced pHi alterations were observed in the tumor region (C). Highly resolved 31P spectra with corresponding pHi values for the indicated voxels (white box) are shown for all participants (A4, B4, C4) with respect to both inorganic phosphate peaks (Pi, ePi) for pHi quantifications. ePi = extracellular inorganic phosphate, Pi = intracellular inorganic phosphate, PCr = phosphocreatine.

Whole-brain intracellular pH (pHi) images in participants with high- and low-grade glioma obtained using phosphorous 31 (31P) 7.0-T spectroscopic MRI. (A, B) Clinical (3.0 T) contrast-enhanced T1-weighted magnetization-prepared rapid-acquisition gradient-echo images with overlays of the volumetric three-dimensional (3D) color-coded pHi map in all three planes in a 75-year-old man (A, study participant 8 in Table 1) and a 68-year-old man (B, study participant 3 in Table 1), both with histopathologically proven glioblastoma (World Health Organization [WHO] grade IV). Axial (A1, B1), sagittal (A2, B2), and coronal (A3, B3) planes show an alkaline shift of pHi in the whole-tumor volume (A, pHi = 7.106 ± 0.045; B, pHi = 7.080 ± 0.059) compared with normal-appearing white matter (A, pHi = 6.996 ± 0.002; B, pHi = 6.993 ± 0.004). (C) Clinical (3.0 T) fluid-attenuated inversion recovery images with overlays of the volumetric 3D color-coded pHi maps in a 34-year-old man (study participant 10 in Table 1) with histopathologically proven astrocytoma (WHO grade II) in axial (C1), sagittal (C2), and coronal (C3) planes. No pronounced pHi alterations were observed in the tumor region (C). Highly resolved 31P spectra with corresponding pHi values for the indicated voxels (white box) are shown for all participants (A4, B4, C4) with respect to both inorganic phosphate peaks (Pi, ePi) for pHi quantifications. ePi = extracellular inorganic phosphate, Pi = intracellular inorganic phosphate, PCr = phosphocreatine.