Fig. 5.

Improvement in meningeal lymphatics attenuates sepsis-induced neuronal damage and neuroinflammation in aged mice. A Immunofluorescence staining was used to detect NeuN (green) in the hippocampus. B Fluorescent intensity of NeuN in the hippocampus, n = 6. C The expression of caspase-3 and PSD-95 in the mouse hippocampus was detected by western blotting using specific antibodies. D, E Expression of caspase-3 and PSD-95 was quantified and normalized to GAPDH levels. Each value was then expressed relative to the control, which was set to 1; n = 4. F Immunofluorescence staining was used to detect Iba1 (red) in the hippocampus. G Quantitative results of the percentage of Iba1-positive area in the total area of the image, n = 6. H, I Levels of Il-1β and IL-6 in the hippocampus, n = 6. **P < 0.01, eGFP + LPS vs. eGFP + vehicle. ^#P < 0.05, ^##P < 0.01, VEGF-C + LPS vs. eGFP + LPS. All data are expressed as the mean ± SD