Fig. 3.

Cordycepin adjuvanted vaccine enhances both Th1 and Th2 response. Mice (n = 5/group) were injected with PBS, vaccine (V) or vaccine adjuvanted with cordycepin (V + C) for 3 times with an interval of 7 days and splenocytes were harvested 14 days after the last injection. (a) Total lymphocyte proliferation activity was measured using CCK-8 methods. Nonspecific proliferation in response to ConA was used as positive control. (b) CD4⁺ T cells were isolated to measure the percentage of cytokine production cells using ELISAPOT assays. Results are presented as the mean ± S.D. ∗, represents p < 0.05 compared with PBS group and #, represents p < 0.05 compared with vaccine group.