Fig 3.

Alpha-diversity indices obtained from different DNA extraction methods after depletion of bovine DNA. Boxes from A to D represent four different alpha-diversity measures. The median value is shown as a line within the box. Whiskers extend to the most extreme value within 1.5 times the interquartile ranges (1.5*IQR). Statistical differences between groups were analyzed by analysis of variance (ANOVA) followed by Tukey’s test (n = 3/group). Different letters indicate significant differences between groups. ML, MolYsis complete5 kit; ML-NM, MolYsis complete5 kit performed with NEBNext Microbiome DNA Enrichment kit; PF, DNeasy PowerFood Microbial kit; PF-NM, DNeasy PowerFood Microbial kit performed with NEBNext Microbiome DNA Enrichment kit.