TABLE 2.
Edman sequencing of mutacin 1140 derivatized with ethanethiol
| Cycle | Residue predicted from DNA sequence | Identified residue |
|---|---|---|
| 1 | Phe | Phe |
| 2 | Lys | Lys |
| 3 | Ser | S-ECa |
| 4 | Trp | Trp |
| 5 | Ser | S-EC |
| 6 | Leu | Leu |
| 7 | Cys | S-EC |
| 8 | Thr | β-M-S-ECa |
| 9 | Pro | Pro |
| 10 | Gly | Gly |
| 11 | Cys | S-EC |
| 12 | Ala | Ala |
| 13 | Arg | Arg |
| 14 | Thr | β-M-S-EC |
| 15 | Gly | Gly |
| 16 | Ser | S-EC |
| 17 | Phe | Phe |
| 18 | Asn | Asn |
| 19 | Ser | S-EC |
| 20 | Tyr | Tyr |
| 21 | Cys | NDb |
| 22 | Cys | ND |
a
Thioethyl cysteine (S-EC) derived from ethanethiol treatment of lanthionine, 3-methyllanthionine, and 2,3-didehydroalanine; β-methylthioethyl cysteine (β-M-S-EC) derived from ethanethiol treatment of 3-methyllanthionine and 2,3-didehydrobutyrine according to the scheme of Meyer et al. (17).
b
ND, not detected.