Skip to main content
. 2023 Dec 19;28(2):e18055. doi: 10.1111/jcmm.18055

FIGURE 4.

FIGURE 4

Compound C blocked asiaticoside from activating 5′ adenosine monophosphate‐activated protein kinase/nuclear factor‐erythroid 2‐related factor 2 and up‐regulating autophagy in diabetes cardiomyopathy injury. (A) Atg5, Beclin1, p‐AMPK, AMPK, nuc‐Nrf2, cell‐Nrf2, HO‐1 and GAPDH (internal reference). (B) LC3B immunofluorescence representative image. (C, D) Semi‐quantitative analysis of Atg5 and Beclin1. (E–G) Semi‐quantitative analysis of p‐AMPK, nuc‐Nrf2 and HO‐1. (H) Semi‐quantitative analysis of LC3B immunofluorescence intensity. The data are expressed as mean ± standard deviation (n = 6). ASI, asiaticoside; CC, compound C; cyto Nrf2, cytoplasmic Nrf2; DCM, diabetes cardiomyopathy; GAPDH, 3‐phosphate glyceraldehyde dehydrogenase; Nuc‐Nrf2, intranuclear Nrf2; ## p < 0.01 compared with the sham group. **p < 0.01 compared with the DCM group. ^^ p < 0.01 compared with the ASI + DCM group.