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. 2023 Dec 19;28(2):e18055. doi: 10.1111/jcmm.18055

FIGURE 7.

FIGURE 7

ML‐385 attenuates asiaticoside‐induced nuclear factor erythroid 2‐related factor 2 nuclear translocation but has little effect on the activation of 5′ adenosine monophosphate‐activated protein kinase in myocardial diabetic cardiomyopathy injury. (A) Atg5, Beclin1, p‐AMPK, AMPK, nuc‐Nrf2, cell‐Nrf2, HO‐1 and GAPDH (internal reference). (B) LC3B immunofluorescence representative image. (C, D) Semi‐quantitative analysis of Atg5 and Beclin1. (E–G) Semi‐quantitative analysis of p‐AMPK, nuc‐Nrf2 and HO‐1. (H) Semi‐quantitative analysis of LC3B immunofluorescence intensity. The data are expressed as mean ± standard deviation (n = 6). ASI, asiaticoside; cyto Nrf2, cytoplasmic Nrf2; DCM, diabetes cardiomyopathy; GAPDH, 3‐phosphate glyceraldehyde dehydrogenase; ML‐385, Nrf2 inhibitor; Nuc‐Nrf2, intranuclear Nrf2. ## p < 0.01 compared with the sham group. **p < 0.01 compared with the DCM group. ^^ p < 0.01 compared with the ASI + DCM group.