TABLE 1:
Antibodies and Reagents:
| Reagents and antibodies | Catalog number | Company |
|---|---|---|
| Vinculin | V9131 | Millipore Sigma |
| β-actin | A1798 | Millipore Sigma |
| Collagen 1a (COL1A) | ab34710 | Abcam |
| RAPH1 | PA5–110270 | Invitrogen/ThermoFisher |
| Arp2/3 | 07–227-I | Sigma-Aldrich |
| LIMK1 | sc515585 | Santa Cruz Biotechnology |
| SREBP1 | sc13551 | Santa Cruz Biotechnology |
| SREBP2 | sc13552 | Santa Cruz Biotechnology |
| Paxillin | sc365379 | Santa Cruz Biotechnology |
| GAPDH | sc32233 | Santa Cruz Biotechnology |
| FAS | sc-48357 | Santa Cruz Biotechnology |
| Fascin 1 | sc46675 | Santa Cruz Biotechnology |
| SREBP1 | NB100–2215 | NOVUS Biologicals |
| SREBP2 | NBP1–54446 | NOVUS Biologicals |
| SREBP2 | LS-B1609-BCD1–1 | Life Span Bioscience |
| SCAP | 12266–1-AP | Proteintech |
| SCAP | MABS2288 | Sigma-Aldrich |
| Collagen 1a (COL1A) | ABT257 | Sigma-Aldrich |
| HMGCR | PA5–37367 | Invitrogen/ThermoFisher |
| Lamin B1 | PA5–19468 | Invitrogen/ThermoFisher |
| Total-paxillin (T-paxillin) | 2542S | Cell Signaling Technology |
| Phospho-paxillin (P-paxillin) | 69363S | Cell Signaling Technology |
| Total-cofilin (T-cofilin) | 5175S | Cell Signaling Technology |
| Phospho-cofilin (P-cofilin) | 3311S | Cell Signaling Technology |
| Phospho-myosin light chain (p-MLC) | 3671 | Cell Signaling Technology |
| Myosin light chain (MLC) | 3672 | Cell Signaling Technology |
| ACC | 3662S | Cell Signaling Technology |
| Fibronectin | Gift from Dr. Harold Erickson, Duke University | |
| Donkey-anti-goat IgG | 705–035-003 | Jackson ImmunoResearch |
| Donkey-anti-mouse IgG | 715–035-150 | Jackson ImmunoResearch |
| Donkey-anti-rabbit IgG | 715–035-144 | Jackson ImmunoResearch |
| Goat-anti-Mouse IgG Alexa Fluro Plus 488 | A32723 | ThermoFisher |
| Goat-anti-Mouse IgG Alexa Fluor Plus 594 | A32742 | ThermoFisher |
| Goat-anti-Rabbit IgG Alexa Fluor Plus 488 | A32731 | ThermoFisher |
| Goat-anti-Rabbit IgG Alexa Fluor Plus 594 | A32740 | ThermoFisher |
| Phalloidin (Alexa Fluor Plus 488 PHA) | A12379 | Invitrogen/ThermoFisher |
| Phalloidin (Alexa Fluor Plus 647 PHA) | A22287 | Invitrogen/ThermoFisher |
| DAPI | UH2820551 | Sigma-Aldrich |
| Fluoromount-G | E139999 | Invitrogen/ThermoFisher |
| Fatostatin | 341329 | Sigma-Aldrich |