TABLE 3:
Significantly changed lipids in fatostatin-treated HTM cells using lipidomic volcano analysis
| Lipid names | FC | log2(FC) | raw. p-value | -LOG10(p) |
|---|---|---|---|---|
| LPS(O-18:0) | 0.73346 | −0.4472 | 0.013767 | 1.8612 |
| LPS(17:2) | 0.761 | −0.39403 | 0.015029 | 1.8231 |
| [TG(51:7),TG(50:0)]_FA16:0 | 0.79898 | −0.32376 | 0.021917 | 1.6592 |
| [TG(51:7),TG(50:0)]_FA18:0 | 0.84839 | −0.23721 | 0.012095 | 1.9174 |
| [TG(48:6)]_FA16:0 | 0.86119 | −0.2156 | 0.0047502 | 2.3233 |
| Cer(d18:1/25:0) | 0.87544 | −0.19191 | 0.0076135 | 2.1184 |
| Cer(d18:1/26:0) | 0.89366 | −0.1622 | 0.038527 | 1.4142 |
| PS(42:3) | 0.89831 | −0.15472 | 0.0070595 | 2.1512 |
| [TG(55:7),TG(54:0)]_FA14:0 | 0.89844 | −0.15451 | 0.0074426 | 2.1283 |
| [TG(47:4)]_FA16:0 | 0.9026 | −0.14784 | 0.04484 | 1.3483 |
| DG(36:6)_C18:1 | 0.90803 | −0.13919 | 0.019259 | 1.7154 |
| [TG(48:3)]_FA18:1 | 0.9121 | −0.13274 | 0.021998 | 1.6576 |
| DG(36:3)_C18:0 | 0.91483 | −0.12842 | 0.046872 | 1.3291 |
| DG(37:6)_C18:1 | 0.91882 | −0.12214 | 0.0205 | 1.6882 |
| [TG(51:9),TG(50:2)]_FA18:3 | 0.92489 | −0.11265 | 0.002672 | 2.5732 |
| DG(44:8),DG(43:1)_C18:1 | 0.92699 | −0.10938 | 0.014584 | 1.8361 |
| PS(P-34:1) | 0.92853 | −0.10697 | 0.027975 | 1.5532 |
| [TG(45:5)]_FA14:0 | 0.92923 | −0.10589 | 0.026106 | 1.5833 |
| [TG(50:3)]_FA14:0 | 0.93005 | −0.10462 | 0.0077825 | 2.1089 |
| DG(37:6)_C18:2 | 0.93198 | −0.10164 | 0.01922 | 1.7162 |
| [TG(49:3)]_FA18:3 | 0.93231 | −0.10112 | 0.011167 | 1.9521 |
| [TG(38:1)]_FA14:0 | 0.93565 | −0.09596 | 0.038698 | 1.4123 |
| DG(O-40:9),DG(38:2)_C18:1 | 0.93997 | −0.08932 | 0.0032856 | 2.4834 |
| DG(31:2),DG(P-14:0/18:1)_C18:2 | 0.94248 | −0.08546 | 0.043189 | 1.3646 |
| DG(38:3)_C18:2 | 0.94465 | −0.08216 | 0.013449 | 1.8713 |
| [TG(47:2)]_FA18:0 | 0.94859 | −0.07614 | 0.011691 | 1.9322 |
| [TG(49:5)]_FA18:2 | 0.94948 | −0.07479 | 0.044194 | 1.3546 |
| [TG(47:3)]_FA18:2 | 0.95833 | −0.0614 | 0.01122 | 1.95 |
| [TG(52:6)]_FA14:0 | 0.96963 | −0.04449 | 0.015535 | 1.8087 |
| [TG(54:5)]_FA18:3 | 0.97092 | −0.04257 | 0.049176 | 1.3083 |
| [TG(64:13),TG(63:6)]_FA18:1 | 1.0156 | 0.022367 | 0.015558 | 1.808 |
| [TG(66:18),TG(65:11),TG(64:4)]_FA18:2 | 1.0165 | 0.023677 | 0.034418 | 1.4632 |
| [TG(56:8),TG(55:1)]_FA18:3 | 1.0298 | 0.042352 | 0.00057611 | 3.2395 |
| [TG(66:6)]_FA18:0 | 1.0303 | 0.043038 | 0.040248 | 1.3953 |
| [TG(64:16),TG(63:9),TG(62:2)]_FA18:0 | 1.0313 | 0.044472 | 0.0087831 | 2.0564 |
| [TG(62:8),TG(61:1)]_FA14:0 | 1.0352 | 0.049884 | 0.037112 | 1.4305 |
| FA(31:0) | 1.0369 | 0.052214 | 0.003511 | 2.4546 |
| [TG(48:8),TG(47:1)]_FA18:2 | 1.037 | 0.052364 | 0.017224 | 1.7639 |
| [TG(64:11),TG(63:4)]_FA18:3 | 1.0385 | 0.054432 | 0.029844 | 1.5251 |
| [TG(52:10),TG(51:3)]_FA16:1 | 1.051 | 0.071799 | 0.039386 | 1.4047 |
| [TG(42:2)]_FA18:2 | 1.0513 | 0.072178 | 0.016568 | 1.7807 |
| FA(28:3) | 1.0554 | 0.077799 | 0.023569 | 1.6277 |
| SM(d18:1/25:0) | 1.0571 | 0.080177 | 0.04653 | 1.3323 |
| FA(26:2) | 1.0578 | 0.081086 | 0.0062176 | 2.2064 |
| [TG(64:11),TG(63:4)]_FA18:0 | 1.0604 | 0.084554 | 0.046904 | 1.3288 |
| [TG(59:13),TG(58:6)]_FA16:0 | 1.0635 | 0.088755 | 0.026298 | 1.5801 |
| [TG(43:2)]_FA18:1 | 1.0655 | 0.091595 | 0.020429 | 1.6897 |
| [TG(45:2)]_FA14:0 | 1.0747 | 0.10394 | 0.034808 | 1.4583 |
| [TG(56:7),TG(55:0)]_FA16:0 | 1.0757 | 0.10524 | 0.041985 | 1.3769 |
| [TG(56:6)]_FA16:0 | 1.0804 | 0.1115 | 0.017136 | 1.7661 |
| [TG(42:2)]_FA14:0 | 1.0824 | 0.11424 | 0.037315 | 1.4281 |
| [TG(46:4)]_FA18:3 | 1.0921 | 0.12715 | 0.04208 | 1.3759 |
| [TG(47:4)]_FA18:1 | 1.0943 | 0.12999 | 0.032941 | 1.4823 |
| [TG(50:4)]_FA16:1 | 1.0964 | 0.13278 | 0.0081318 | 2.0898 |
| FA(40:6) | 1.0967 | 0.13317 | 0.011971 | 1.9219 |
| [TG(54:5)]_FA16:0 | 1.1035 | 0.14209 | 0.032934 | 1.4824 |
| FA(8:1) | 1.1108 | 0.15154 | 0.022394 | 1.6499 |
| [TG(41:0)]_FA18:0 | 1.1137 | 0.15534 | 0.0016727 | 2.7766 |
| PC(36:3),PC(P-37:2) | 1.1324 | 0.17942 | 0.041981 | 1.377 |
| PE(42:4) | 1.1434 | 0.19338 | 0.036597 | 1.4366 |
| PE(30:0),PE(O-31:0) | 1.1764 | 0.23443 | 0.023423 | 1.6304 |
| DG(33:2)_C18:0 | 1.2035 | 0.2672 | 0.0017644 | 2.7534 |
| PE(35:4),PE(O-36:4),PE(P-36:3) | 1.2087 | 0.27346 | 0.0096295 | 2.0164 |
| FA(14:1) | 1.2135 | 0.27917 | 0.04429 | 1.3537 |
| DG(33:4)_C18:2 | 1.2209 | 0.28789 | 0.0045654 | 2.3405 |
| PC(31:2),PC(O-32:2),PC(P-32:1) | 1.2282 | 0.29658 | 0.048578 | 1.3136 |
| PE(36:7),PE(35:0),PE(O-36:0) | 1.2336 | 0.30282 | 0.0094306 | 2.0255 |
| PE(39:8),PE(O-40:8),PE(P-40:7),PE(38:1),PE(O-39:1),PE(P-39:0) | 1.236 | 0.30568 | 0.032059 | 1.494 |
| SM(d16:1/22:1) | 1.2885 | 0.36569 | 0.0041656 | 2.3803 |
| PC(34:3),PC(P-35:2) | 1.3119 | 0.39163 | 0.023755 | 1.6242 |
| PC(42:8),PC(41:1),PC(O-42:1),PC(P-42:0) | 1.3475 | 0.43025 | 0.032877 | 1.4831 |
| PC(37:6),PC(O-38:6),PC(P-38:5) | 1.3497 | 0.4326 | 0.042341 | 1.3732 |
| PC(42:7),PC(41:0),PC(O-42:0) | 1.3736 | 0.45791 | 0.041498 | 1.382 |
| PC(35:5),PC(O-36:5),PC(P-36:4) | 1.5893 | 0.66842 | 0.0023312 | 2.6324 |
| SM(d16:1/20:1) | 1.6734 | 0.74278 | 0.0090041 | 2.0456 |
| PC(30:3) | 1.6764 | 0.74541 | 0.039106 | 1.4078 |
| PC(32:4) | 1.8924 | 0.92018 | 0.021488 | 1.6678 |
| Cer(d20:0/26:0) | 1.9283 | 0.94735 | 0.0084497 | 2.0732 |
| PC(32:3),PC(P-33:2) | 2.4561 | 1.2964 | 0.0031201 | 2.5058 |
Lipids significantly changed in fatostatin-treated compared to DMSO-treated HTM cells. Columns (left to right) show the lipid name, FC (fold change), log2(FC), raw p-value, and -log10(p) based on lipidomic volcano analysis, where n = 4 (biological replicates). A total of 79 specific lipids were above the threshold (|FC| > 1 and p < 0.05), log2(FC) < 0 indicates decreased lipids in fatostatin-treated HTM cells, whereas log2(FC) > 0 indicates increased lipids in fatostatin-treated HTM cells.