FIG. 3.

Dose dependence of attachment and ingestion of LAM microspheres by MDMs. MDMs were incubated with the indicated number of LAM microspheres for 60 min at 37°C and then fixed with 3.3% formalin. Fixed MDMs were visualized as in Fig. 2, except that the number of total cell-associated microspheres was visualized and enumerated by phase microscopy instead of fluorescence microscopy. The number of microspheres attached to the cell (open circles) or totally associated (solid circles) was measured. The number of internalized microspheres (open squares) was calculated. All points are means ± standard errors from two independent experiments.