Figure 5.
Knockout of CsExo70B compromises pathogen resistance in cucumber. (a, b) CsExo70B knockout lines exhibited reduced reactive oxygen species (ROS) production triggered by flg22 (a) or chitin (b). Data represent the means ± SD (n = 6). (c) CsExo70B knockout lines are more susceptible to Fusarium oxysporum f. sp. Cucumerinum Owen (Foc). Images were taken at 14 days post‐inoculation (dpi), and the red arrows indicated wilted hypocotyls and stems. (d) Disease index (DI) in WT and CsExo70B knockout lines shown in (c). Data are means ± SD (n = 3). (e) The disease resistance phenotypes of CsExo70B knockout lines after inoculation with Pseudomonas syringae pv. lachrymans (Psl). The cucumber plants with two true leaves were spray‐inoculated with Psl, and images were taken at 3 dpi. Scale bar = 1 cm. (f) CsExo70B knockout lines displayed increased susceptibility to Psl. Bacterial population in the leaf was measured 3 days after spray inoculation. Values are means of log (colony‐forming units [CFU] cm−2 leaf tissue) ± SD (n ≥ 28). Statistical significances relative to WT are indicated by asterisks (**P < 0.01, one‐way ANOVA).