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[Preprint]. 2024 Jan 16:2024.01.15.575715. [Version 1] doi: 10.1101/2024.01.15.575715

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Figure 3. — Assessing the top p-S65-Ub antibodies in ELISA using recombinant protein. (A) Sandwich ELISA results for the detection of recombinant K48 (solid line) and K63 (dash line) p-S65-Ub tetramers (Ub4) that were serially diluted to the range of 1–1,000,000 pg/ml. (B) Direct ELISA results for the detection of p-S65-PRKN and nonphosphorylated PRKN recombinant proteins that were serially diluted to the range of 1–1,000,000 pg/ml. N = 2. Data are shown as mean with SD.