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[Preprint]. 2024 Jan 16:2024.01.15.575715. [Version 1] doi: 10.1101/2024.01.15.575715

Figure 4.

Figure 4.

Assessing the top p-S65-Ub antibodies by sandwich ELISA using lysates from cells, mouse brain, and human brain. (A) ELISA detection of p-S65-Ub levels in cell lysates from CCCP or DMSO treated WT and PINK1 KO HEK293E cells. One-way ANOVA. N = 2. Data are shown as mean with SEM. (B) ELISA detection of p-S65-Ub levels in hemibrain lysates from unstimulated WT and pink1 KO mice. Unpaired t-test. N = 6 per group. Data are shown as mean with SEM. (C) ELISA detection of p-S65-Ub levels in human brain lysates from neurological normal controls and AD cases. Mann-Whitney U test. N = 10 per group. Data are shown as median with interquartile range. * p<0.05, ** p<0.005, *** p<0.0001. Asterisks (*) indicate the comparison to the untreated WT or the comparison between two groups under the brackets. Ref. Ab - reference antibody, WT - wild-type, KO - knockout, AD - Alzheimer disease, n.s. - non significant.