Table 1.
LoB, LoD, and LoQ of p-S65-Ub MSD ELISA with recombinant protein
| ref. Ab | 11H2K3 | 19H1K1 | 29H2K2 | 30H3K1 | 41H1K3 | |
|---|---|---|---|---|---|---|
|
| ||||||
| LoB (MSD-ECL [AU]) | 469 | 1162 | 522 | 774 | 578 | 2340 |
| LoD for K48 p-S65-Ub4 (MSD-ECL [AU]) | 472 | 1268 | 538 | 860 | 590 | 2454 |
| LoD for K63 p-S65-Ub4 (MSD-ECL [AU]) | 473 | 1178 | 522 | 804 | 912 | 2349 |
| LoQ for K48 p-S65-Ub4 (fg/ml) | 1x104 | 1x106 | 100 | 1x107 | 1x105 | 1x106 |
| LoQ for K63 p-S65-Ub4 (fg/ml) | 1x106 | 1x107 | 1x106 | 1x107 | 1x107 | 1x105 |
| Chain preference | K48 >> K63 | K48 >> K63 | K48 >> K63 | K48 = K63 | K48 >> K63 | K48 << K63 |
ref.Ab - reference antibody, LoB - Limit of Blank, LoD - Limit of Detection, LoQ - Limit of Quantitation, Ub - ubiquitin tetramer, K48 - polyubiquitin chain linked by lysine residue 48, K63 - polyubiquitin chain linked by lysine residue 63.