Table 2.
Comparison of MSD ELISA detection of p-S65-Ub with different testing materials
Fold change in MSD ELISA detection | ref. Ab | 11H2K3 | 19H1K1 | 29H2K2 | 30H3K1 | 41H1K3 |
---|---|---|---|---|---|---|
| ||||||
HEK293E cells baseline detection (WT vs. PINK1 KO) | 2.1 | 1.3 | 1.8 | 1.1 | 1.4 | 1.1 |
HEK293E cells stressed condition (treated WT vs. PINK1 KO) | 143.5 | 52 | 91.2 | 5.6 | 80.2 | 4.8 |
Mouse brain baseline detection (WT vs. pinkl KO) | 1.5 | 1.1 | 1.6 | 1 | 1.2 | 1 |
Human brain disease condition (control vs. AD) | 9.3 | 10.8 | 2.6 | 4.9 | 3.7 | 2 |
WT - wild-type, KO - knockout, AD - Alzheimer disease.