Figure 6. Biologically relevant weak acids inhibit growth of the alr1 mutant.

Molecular docking of (A) lactate (B) propionate and (C) itaconate to the ATP binding site of Ddl. (D) The relative positions and poise of different organic anions in relation to acetate in the d-Ala binding site of Ddl was determined using Schrödinger Glide. The growth (OD₆₀₀) of the WT and alr1 mutant in TSB containing (E) lactic acid (40 mM) (F) propionic acid (20 mM) and (G) itaconic acid (20 mM) in the presence or absence of 5 mM d-Ala.