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[Preprint]. 2024 Jan 21:2024.01.20.576352. [Version 1] doi: 10.1101/2024.01.20.576352

Figure 4. Obelisks form a self-consistent set.

Figure 4.

Predicted Obelisk secondary structures depicted as “jupiter” plots where chords represent predicted basepairs (coloured by basepair probability from 0, grey, to 1, red, see methods) with predicted open reading frames (ORFs, preceded by predicted Shine-Delgarno sequences, purple) depicted: Oblin-1 (green), Oblin-2 (yellow, based on blastp hits against the Oblin-2 consensus), and “2ndORF” (orange). Obelisk-ɣ’s suggested CRISPR spacer match illustrated in light blue. ColabFold predictions of Oblin-1 tertiary “globule” structures built with ad hoc multiple sequence alignment (MSA) construction (coloured cartoons) superimposed over the RDVA-derived MSA prediction for Obelisk-α where possible (black line, Figure 2a, see methods). Prediction confidence (pLDDT) shown as cartoon colouring as in Supplementary Figure 3. Greek letter key: α : alpha, β : beta, ɣ : gamma, δ : delta, ε : epsilon, ζ : zeta, η : eta, θ : theta, ι: iota, κ : kappa, λ : lambda, μ : mu, ν : nu, and ξ : xi.