Figure 6. Postnatal Cbfβ deficiency in cartilage resulted in increased Wnt signaling, inflammatory genes expression, decreased cartilage formation genes expression in the knee articulate cartilage.

(A-E) IHC staining of (A) anti-Cbfβ, (B) anti-Col2α1, (C) anti-Adamts5, and (D) anti-Mmp13 of hip joint from 2-month-old male Cbfβ^f/fCol2α1-CreER^T mice. (E) Negative control of (A-D). (F) Quantification for (A). (G) Quantification for (B-D). (H-I) IF staining of (H) anti-Cbfβ and (I) Active-β-catenin of knee joint from 3-month-old male Cbfβ^f/fAggrecan-CreER^T mice. (J-K) Quantification of (H) and (I). (L-M) IF staining of (L) anti-Sox9, and (M) anti-Dkk1 of knee joint from 4.5-month-old male Cbfβ^f/fAggrecan-CreER^T mice with oil injection or TMX injection. (N-O) Quantification of (L) and (M). Data are shown as mean ± SD. n= 3. *p < 0.05, **p < 0.01, ***p < 0.001.