TABLE 2.
Comparison of bovine, human, and ovine inocula in Tg mice expressing native and chimeric BoPrP genes
| Transgenic line | PrP expression (fold) | Inoculum | Mean incubation period ± SEM (days) | n/n0a |
|---|---|---|---|---|
| BoPrP 4125 | 8-16 | BSE | 240 ± 5.3 | 41/41 |
| vCJD | 270 ± 5.2 | 77/77 | ||
| Scrapie | 225 ± 9.4 | 48/48 | ||
| Bo3M(HVEII)7177 | 16-32 | BSE | 254 ± 7.5 | 10/10 |
| vCJD | 304 ± 13.4 | 7/7 | ||
| Scrapie | 317 ± 10.4 | 9/9 | ||
| Bo3M(HVEII)7187 | 8-16 | BSE | 235 ± 5.0 | 9/9 |
| vCJD | 273 ± 25.6 | 8/8 | ||
| Scrapie | 325 ± 19.0 | 7/7 | ||
| Bo3M(HVEIV)14926 | 8 | BSE | >580 | 0/9 |
| vCJD | >580 | 0/9 | ||
| Scrapie | >648 | 1/7* | ||
| Bo3M(YIQII)23939 | 8 | BSE | 248 ± 7.8 | 6/6 |
| vCJD | 277 ± 7.4 | 9/9 | ||
| Scrapie | 449 ± 8.0 | 7/7 | ||
| Bo3M(YIQII)23953 | 8 | BSE | 232 ± 3.6 | 17/17 |
| vCJD | 255 ± 6.9 | 10/10 | ||
| Scrapie | 347 ± 8.2 | 19/19 | ||
| Bo3M(YIQVV)12138 | 8 | BSE | 260 ± 13.5 | 10/10 |
| vCJD | 226 ± 8.0 | 8/8 | ||
| Scrapie | >502 | 1/9** | ||
| Bo3M(YIQIV)10667 | 8-16 | BSE | 351 ± 34.1 | 5/5 |
| vCJD | 319 ± 16.2 | 6/6 | ||
| Scrapie | 514 ± 71.5 | 6/6 | ||
| Bo3M(HIQVV)29285 | 8 | BSE | 432 ± 12.0 | 6/6 |
| vCJD | 437 ± 9.8 | 9/9 | ||
| Scrapie | >655 | 1/6*** |
a
n, number of ill animals; n0, number of inoculated animals. *, one animal diagnosed with prion disease at 600 days; **, one animal diagnosed with prion disease at 483 days; ***, one animal diagnosed with prion disease at 603 days.