Figure 2:

Analysis of human and mouse datasets show significant enrichment of known Ca²⁺-regulated processes, proteins and pathways.

(A,B) Gene set enrichment analysis (GSEA) of CRPs from human (A) and mouse (B) datasets show significant enrichment of molecular function terms related to Ca²⁺ binding and signaling (green text).

(C) GSEA of proteins with a significant thermal stability change in response to Mg²⁺ addition shows an enrichment for DNA, RNA and nucleotide binding molecular functions, and lack Ca²⁺-related processes.

(D) Ion-induced thermal stability changes of Annexin proteins from human and mouse datasets show a Ca²⁺-specific response.

(E) GSEAof human dataset using KEGG annotations enriches Ca²⁺ signaling.

(F) Schematic representation of the Ca²⁺ regulated Gq signaling pathway. Proteins shown were quantified in the human lysates. Bolded proteins are identified as CRPs.

(G) Ion-induced thermal stability changes of Gq pathway proteins, relative to EGTA condition. Dotted lines show log₂(FC) ± 0.2. Error bars: standard deviation of replicate measurements.