Figure 4.

CYS6 interacts with XCP1. A) Split-luciferase assays were performed on XCP1 and CYS6. Different combinations of Agrobacteria carrying nLuc, cLuc, XCP1-nLuc, or cLuc-CYS6 as indicated were infiltrated into N. benthamiana leaves, and luciferin was sprayed onto the infiltrated leaves 3 d later to detect luminescence signals with a CCD camera. B) Purified recombinant GST and GST-CYS6 were used to pull down XCP1-HIS from cell lysates. The protein levels of XCP1-HIS, GST, and GST-CYS6 were detected by immunoblots using anti-HIS and anti-GST antibodies. C)XCP1-cYFP and CYS6-nYFP were coexpressed in N. benthamiana leaves together with the vacuolar marker γ-TIP-mCherry for 3 d. The YFP and mCherry signals were visualized using a confocal microscope. Scale bar, 20 µm.