Figure 8.

XCP1 interacts with RBOHD. A) Split-luciferase assays were performed to test the interaction of XCP1 and RBOHD. Different combinations of Agrobacteria containing nLuc, cLuc, RBOHD-nLuc, or cLuc-XCP1 constructs as indicated were infiltrated into N. benthamiana leaves, and luciferin was sprayed onto the infiltrated leaves 3 d later to detect luminescence signals with a CCD camera. B) Purified recombinant MBP, MBP-RBOHD-N, and MBP-RBOHD-C were used to pull down XCP1-HIS from cell lysates. The protein levels of XCP1-HIS, MBP, MBP-RBOHD-N, and MBP-RBOHD-C were detected by immunoblots using anti-HIS and anti-MBP antibodies. TM, transmembrane region. C) Different combinations of Agrobacteria carrying nYFP, cYFP, XCP1-cYFP, or RBOHD-nYFP constructs as indicated were infiltrated into N. benthamiana leaves. YFP signals were visualized 3 d later using a confocal microscope. Scale bar, 20 µm.