Figure 9.

XCP1 depends on RBOHD to repress elf18-triggered defenses. A) Mutating RBOHD suppresses the enhanced elf18-triggered resistance phenotype of xcp1. Experiments were performed as described in Fig. 2A. Significant differences were detected by 2-way ANOVA. Data are shown as means ± Sd (n = 8). B) and C) Mutating XCP1 abolishes the enhanced ROS production of the xcp1 mutant. Experiments were performed as shown in Fig. 2C. Significant differences were detected by Student’s t test. Data are shown as means ± Sem (n = 8). D) Mutating RBOHD does not affect the elf18-triggered phosphorylation of MPK3 and MPK6. Experiments were performed as described in Fig. 2B. E) Presence of putative orthologs for CYS6, XCP1, and RBOHD in different species. Tick marks suggest that the species possess the indicated related proteins. The phylogenetic tree was generated by Phytozome. *P < 0.05; **P < 0.01; ****P < 0.0001; ns, no significant difference.