FIG. 5.

Dengue virus RNA replication inhibits STAT1 and STAT2 phosphorylation in response to IFN-α and reduces steady-state levels of STAT2. (A) K562, K562.ΔCprME-PAC2A, and cured K562 cells; (B) THP-1 and THP-1.ΔCprME-PAC2A cells. Cells were left untreated or treated with 100 IU of IFN-α or IFN-γ/ml for 30 min and then lysed in SDS loading buffer. Proteins were separated by SDS-PAGE and then analyzed by immunoblotting with specific antibodies for STAT1, phosphorylated STAT1, STAT2, and phosphorylated STAT2, as indicated.