FIG. 3.

Analysis of recombinant BMV RNA1 progeny in doubly infected barley host. Two pairs of parental RNA1 mutants designated as P1 through P4, corresponding to O3-RNA1/O6-RNA1 (A) and O6-RNA1/O8-RNA1 (B) pairs, and the projected RNA1 recombinants (designated as R1 through R4) are shown schematically on the left. All the elements are as described in the legends to Fig. 1 and 2. The tables on the right show the distribution of the parental and recombinant cDNA clones in the progeny RNA1 variants as they were identified in total RNA extracts from the whole leaf tissue, as well as the oRF. Parental molecules and recombinants are labeled, and vertical arrows represent marker restriction sites. The experimental methodology used was similar to that for RNA2, as described in the legend to Fig. 2.