TABLE 1.
Sequences of the primers used
| Primer or primer pair | Sequence from 5′ to 3′ enda |
|---|---|
| R2A | CATGCCTGCAGGTCGAC |
| R2B | TTTTCGAAGACATCTTGGGGATCCTAGAAAG |
| R2G | GGAGGCCTAACGTCAGTTGATGC |
| R2H | CACCCGCATTCTAAGACTTGCTATGCAAGCCCATGCAGGATCCCCTAAGG |
| AN1a | GGTTCAATCCCTTTTCGAACACGGTTCTGCTAC |
| AN1b | GTAGCAGAACCGTGTTCGAAAAGGGATTGAACC |
| AN7 | GGAGAGCCCTGTTCGAAGTAGGAACGTTGTGG |
| AN8 | CCACAACGTTCCTACTTCGAACAGGGCTCTCC |
| 139 | GTAAACCACGGAACGAGGTTC |
| R2B2 | GAACCATTTGTTGGACGGTGTCGCAAATGGATCC |
| R2C | TTCCATGGACTTTGATAGGATCCTAGAAAG |
| R2D | CTGGTACCGACATAAATCAGTC |
| R2E | TGACGCGTTTACATATTTCGGTAATACTCTGGTCACCATGGC |
| R2F | GGGAGCTCCTTAGTACTACAG |
| R2G2 | GGAGGCCTAACGTCAGTTGATGCATTG |
| 1st | CAGTGAATTCTGGTCTTTTTAGAGATTTACAG |
| ANB/ANC | GAAAATCAACGTTCGAAATAAGCTCTCTATTG CAATAGAGAGCTTATTTCGAACGTTGATTTTC |
| R1A/R1B | GTGCCGCATGCGAAAGATCTTGCAAGAAAGCGATG CATCGCTTTCTTGCAAGATCTTTCGCATGCGGCAC |
| AN3 | CACTGTGTATGGTTCGAAGACATATCTAAG |
| AN4 | CTTAGATATGTCTTCGAACCATACACAGTG |
| AN5 | CCGTATGGGAGAAGATCTAATTGTGACGGCG |
| AN6 | CGCCGTCACAATTAGATCTTCTCCCATACGG |
| R1E | GTTTCTGACTCGTCTTTCGAACGTTGAAGAATTTG |
| R1F | CAAATTCTTCAACGTTCGAAAGACGAGTCAGAAAC |
| R1G | CGGCGAGCTCGTCGGAGATCTGATCTTTAATTGTG |
| R1H | CACAATTAAAGATCAGATCTCCAGCGAGCTCGCCG |
| 119 | CGGTCACTATTGAAGAGC |
| AN1.3 | GTAGTACGCGTACTGCATGC |
| AN4.1 | CAAACGTGATATCGGTACAG |
| AN5.1 | CTGTACCGATATCACGTTTG |
| seq3.2 | GGACTCAGGGCTCAACTC |
a
Marker restriction sites are underlined and nucleotide substitutions are in bold type. Pairs of primers used in PCR with Pfu DNA polymerase have both sequences on one line.