TABLE 2.

Characterization of the infectivity and stability of M- RNA2-BMV and O- RNA1-BMV mutants, as determined for the C. quinoa host^a

BMV mutant	Location of mutation	Marker(s)c	No. of local lesions	Days until symptom developmentd	Stability or infectivity of mutationse
wt	n/ab	n/a	36	9	n/a
M1- RNA2-BMV	RNA2 5′ UTR	M1-93 BamHI	33	9	Unstable
M2- RNA2-BMV	RNA2 ORF	M2-326 BamHI	27	10	Stable
M34- RNA2-BMV	RNA2 ORF	M3-897 BamHI
		M4-1708 BstEII	31	9	Stable
M5- RNA2-BMV	RNA2 3′ UTR	M5-2780 BamHI	37	9	Unstable
M6- RNA2-BMV	RNA2 ORF	M6-2517 NsiI	22	11	Stable
M1345-RNA2-BMV	RNA2 5′ UTR, ORF, 3′ UTR	M1-93 BamHI			M1-unstable
		M3-897 BamHI	29	10	M3-stable
		M4-1708 BstEII			M4-stable
		M5-2780 BamHI			M5-unstable
O1- RNA1-BMV	RNA1 5′ UTR	O1-30 BstBI	35	9	Unstable
O2- RNA1-BMV	RNA1 ORF	O2-217 BstBI	0	10	Not infectious
O3- RNA1-BMV	RNA1 ORF	O3-511 BglII	28	10	Stable
O4- RNA1-BMV	RNA1 ORF	O4-990 BstBI	25	9	Stable
O5- RNA1-BMV	RNA1 ORF	O5-2183 BglII	0	10	Not infectious
O6- RNA1-BMV	RNA1 ORF	O6-1472 BstBI	27	10	Stable
O7- RNA1-BMV	RNA1 3′ UTR	O7-3004 BstBI	38	9	Unstable
O8- RNA1-BMV	RNA1 ORF	O8-2933 BglII	24	10	Stable

^aSince BMV carries the tripartite RNA genome, the host plants were coinoculated with equal amounts of the mutant RNA segment plus the remaining two wt BMV RNAs, all synthesized by in vitro transcription. See Materials and Methods for further details.

^bn/a, not applicable.

^cNewly created restriction site.

^dThe numerals show the number of days postinoculation necessary for symptom appearance.

^eThe stability of marker mutations was determined by restriction analysis of 24 cDNA clones and sequencing of 3 to 5 cDNA clones obtained from total RNAs combined from 3 to 4 C. quinoa leaves per mutation.