FIG. 3.

Effect of E3-gp19 deletion on major histocompatibility complex class I expression. Hep3B tumor cells were infected with the E2F/E3+, E2F/hGM/E3b, or E2F/E3Δgp19 virus at 100 particles per cell and 1,000 particles per cell for 2 h, after which the infection medium was aspirated and cells were cultured in fresh complete medium for 24 h. The major histocompatibility complex class I expression level was measured by staining cells with a pan-class I monoclonal antibody and analyzed by fluorescence-activated cell sorting analysis. Each column represents the average of triplicates ± standard deviation. *, P < 0.001 compared with E2F/E3+-infected cells, one-way analysis of variance.